电离辐射通过转变成生长因子-β-介导的上皮-间质转换来促进癌细胞的侵袭迁移

2022-02-21 06:44 来源:乌兰察布妇科医院

Int J Radiat Oncol Biol Phys 2011 Dec;81 (5): 1530-7. [IF:4.503]Ionizing radiation promotes migration and invasion of cancer cells through transforming growth factor-Beta-mediated epithelial-mesenchymal transition.Zhou YC , Liu JY , Li J , Zhang J , Xu YQ , Zhang HW , Qiu LB , Ding GR , Su XM , Mei-Shi , Guo GZ .Department of Radiation Oncology, Xijing Hospital Fourth Military Medical University, Xi'an, China; Department of Radiation Medicine, College of Preventive Medicine, Xijing Hospital Fourth Military Medical University, Xi'an, China.第四军医大学西京医院放射科

AbstractTo examine whether ionizing radiation enhances the migratory and invasive abilities of cancer cells through transforming growth factor (TGF-β)-mediated epithelial-mesenchymal transition (EMT). Six cancer cell lines originating from different human organs were irradiated by (60)Co γ-ray at a total dose of 2 Gy, and the changes associated with EMT, including morphology, EMT markers, migration and invasion, were observed by microscope, Western blot, immunofluorescence, scratch assay, and transwell chamber assay, respectively. Then the protein levels of TGF-β in these cancer cells were detected by enzyme-linked immunosorbent assay, and the role of TGF-β signaling pathway in the effect of ionizing radiation on EMT was investigate by using the specific inhibitor SB431542. After irradiation with γ-ray at a total dose of 2 Gy, cancer cells presented the mesenchymal phenotype, and compared with the sham-irradiation group the expression of epithelial markers was decreased and of mesenchymal markers was increased, the migratory and invasive capabilities were strengthened, and the protein levels of TGF-β were enhanced. Furthermore, events associated with EMT induced by IR in A549 could be reversed through inhibition of TGF-β signaling. These results suggest that EMT mediated by TGF-β plays a critical role in IR-induced enhancing of migratory and invasive capabilities in cancer cells.

摘要 :阐述辐射源是否可通过转化生长因子-β(TGF-β)-依赖性的上皮-泌尿系统转换 (EMT)来促进抗体的侵袭移至。应用于总量2Gy(60)Coγ线照射源自人类器官的6种抗体,记录与EMT之外的变化,这包括分别依靠显微镜系统设计,蛋白质;还有方法,免疫荧光系统设计,污渍试验和Transwell小室试验来观察并检测细胞组织型态,EMT标识,侵袭移至战斗能力等。引入酶联免疫吸附法检测这些抗体中会TGF-β蛋白总体,依靠特别抑制剂SB431542来指标TGF-β频率通路在辐射源EMT中会的效用。经过总量为2Gy照射的抗体中会长期存在间叶细胞的表达,与骗照射组相比较其上皮标识减少,间叶细胞标识缩减,同时其侵袭转移战斗能力增强,TGF-β蛋白总体也提高。进一步发现由A549辐射源可借的EMT可通过对TGF-β频率抑制牵涉到逆转。这些相比之下TGF-β依赖性的EMT在辐射源可借增强抗体侵袭转移战斗能力中会起着关键效用。

撰稿人: lizexiu

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